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undergraduate thesis
Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura
završni rad
Split: University of Split, Faculty of Chemistry and Technology, 2019. urn:nbn:hr:167:251531
Alerić, Anđelo
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Alerić, A. (2019). Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:251531

Alerić, Anđelo. "Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:251531

Alerić, Anđelo. "Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:251531

Alerić, A. (2019). 'Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 26 May 2022, https://urn.nsk.hr/urn:nbn:hr:167:251531

Alerić A. Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2019 [cited 2022 May 26] Available at: https://urn.nsk.hr/urn:nbn:hr:167:251531

A. Alerić, "Apoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:167:251531

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TitleApoptotičko djelovanje određenih biološki aktivnih spojeva na TCCSUP staničnu liniju karcinoma mokračnog mjehura : završni rad
Title (english)Apoptotic effect of selected biologically active compounds on TCCSUP urinary bladder cancer cell line : bachelor thesis
AuthorAnđelo Alerić
MentorMila Radan (mentor)
MBZ: 247111
Committee memberOlivera Politeo (predsjednik povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2019-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Cilj ovog završnog rada bio je ustanoviti apoptozu induciranjem pomoću odreĊenih spojeva (timokinon, farnezol, nerolidol, tujopsen i fitol) na TCCSUP staniĉnu liniju humanog karcinoma mokraćnog mjehura pomoću protoĉne citometrije. Apoptoza ili programirana smrt stanice je jako vaţan fiziološki proces u oĉuvanju homeostaze. Istraţivanja apoptoze su veoma popularna u opisivanju djelovaja mnogih biološki aktivnih spojeva. Danas postoje mnoge razliĉite analitiĉke metode i tehnike kojima se koriste znanstvenici za praćenje svakog koraka apoptoze, to su na primjer protoĉna citometrija, fluorescentna mikroskopija i dr. Rad se sastoji od eksperimentalnog dijela u kojem se TCCSUP korištena staniĉna linija tretirana s otopinama odabranih spojeva. Nadalje stanice su se bojale specifiĉnim fluorokromima. Primarno bojilo je antikoagulacijski Ca 2+ -ovisan protein aneksin V konjugiran sa fluorescein izotiocijanatom. U ranoj apoptozi taj se konjugat, reverzibilno veţe za fosfatidilserin, koji je translociran sa unutarnjeg sloja na vanjski sloj staniĉne membrane dajući zelenu fluorescenciju. U kasnoj apoptozi, staniĉna membrana postane propusna za sekundarno bojilo propidij-jodid koji se veţe za DNA, dajući crvenu fluorescenciju. Korištenjem protoĉne citometrije odreĊeno je koliko je stanica ţivo, u apoptotozi i/ili nekrotiĉno.
Abstract (english)
The aim of this thesis was detection of apoptosis induced by selected biologically active compounds (thymoquinone, farnesol, nerolidol, thujopsene and phytol) on the bladder cancer (TCCSUP) cell line using flow cytometry. Apoptosis or programmed cell death is very important physiological procesin protection of homeostasis. Research of apoptosis, has been very popular in detailing and describing an effects of all kinds of different biologically active compounds. Today, there's many different analitical methods and techniques scientist can use for monitoring every step in programmed cell death (PCD), like for example, flow cytometry, flourescent microscopy etc. The thesis is consistig of experimetal part in which TCCSUP cancer cell line is treated with solutions of the selected compounds. Afterwards cells were stained with specific fluorochromes. Primary staining agent is anticoagulation, Ca 2+ -dependent protein annexin V conjugated wih fluorecsein isothiocyanate (FTIC). During early apoptosis this conjugate reversibly binds with phosphatidylserine, that is transloceted from inner sheet to outer sheat of cell membrane giving a green fluorescence. In late apoptosis cell membrane becomes permeable for secondary staining agent propidium iodide (PI) and it binds for DNA giving a red fluorescence. Then, flow cytometry was used to determine how much cells are viable, apoptotic and/or necrotic, in every sample.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:251531
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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RepositoryRepository of the Faculty of chemistry and technology
Created on2020-01-30 11:08:51