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master's thesis
Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats
Split: University of Split, School of Medicine, 2018. urn:nbn:hr:171:526457
Hauke, Tim Julian
University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

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Hauke, T. J. (2018). Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:526457

Hauke, Tim Julian. "Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats." Master's thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:526457

Hauke, Tim Julian. "Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats." Master's thesis, University of Split, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:171:526457

Hauke, T. J. (2018). 'Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats', Master's thesis, University of Split, School of Medicine, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:171:526457

Hauke TJ. Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats [Master's thesis]. Split: University of Split, School of Medicine; 2018 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:171:526457

T. J. Hauke, "Expression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats", Master's thesis, University of Split, School of Medicine, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:171:526457

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TitleExpression pattern of apoptosis-inducing factor in the kidneys of streptozotocin-induced diabetic rats
Title (croatian)Izražaj čimbenika indukcije apoptoze (AIF) u bubrezima štakora sa streptozotocin-induciranim dijabetesom
AuthorTim Julian Hauke
MentorKatarina Vukojević (mentor)
GranterUniversity of Split
School of Medicine
(Department of Anatomy, Histology and Embryology)
Split
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Objectives: The aim of this study is to investigate the significance of AIF expression in the development of DKD by observing the expression of AIF in 2 weeks and 2 months diabetic kidney samples and then comparing them to healthy rat kidneys of the same age. Materials and methods: Diabetes mellitus (DM) was induced by i/p injecting 55 mg/kg streptozotocin (STZ) to male Sprague-Dawley rats, and was validated by measuring blood glucose level. Control group received citrate buffer. Animals were sacrificed after 2 weeks and 2 months. Cortex areas (glomeruli, proximal and distal tubules) and medullary areas (collecting ducts and distal tubules) were analyzed with antibodies raised against AIF. Results: AIF expression is located in the cortex as well as in the medulla of the kidney. In the cortex AIF is mainly present in the proximal convoluted tubules (pct) and in the medulla mainly present in the collecting ducts (cd). The highest percentage is present in the 2 weeks DM pct group with 92% positive staining. The cd shows a very mild incline in AIF positivity form 81% in the 2 weeks control group to 89% in the 2 month DM group. The staining intensity rose from moderate to strong staining. The pct showed a similar incline and the staining intensity increased stronger from mild to strong. The same rise in intensity was observed in the distal convoluted tubules but the relative increase in AIF positivity was stronger. What is striking is that the incline and absolute AIF staining intensity is roughly the same from the 2 weeks to 2 month in the control group and the DM group, that there is no big difference between the 2 weeks and 2 month DM groups. The absolute amount of AIF cell positivity is also roughly equal in the DM groups and in the DM 2 week group (38%) even twice as big as in the DM 2 month group (18%). Conclusions: Since AIF has a dual role, for the future it is important, if we want to compare studies to find a method to differentiate AIF in different states. If we manage this, AIF could become a parameter to assess kidney status in DKD and one day becomes a tool to block progression of DKD.
Abstract (croatian)
Ciljevi: Cilj ovog istraživanja bio je istražiti značaj AIF ekspresije u razvoju dijabetička bolest bubrega (DBB) promatranjem ekspresije AIF u 2 tjedna i 2 mjeseca bubrega bubrega, a zatim usporedbom sa zdravih bubrega štakora iste dobi. Materijali i metode: Diabetes mellitus (DM) je induciran i/p injektiranjem 55 mg/kg streptozotocina (STZ) mužjacima Sprague-Dawley štakora i validiran mjerenjem razine šećera u krvi. Kontrolne životinje su primale citratni pufer. Životinje su žrtvovane nakon 2 tjedna i 2 mjeseca od indukcije dijabetesa ili citratnog pufera. Područja kore (glomeruli, proksimalni i distalni tubuli) i područja srži (sabirni kanali i distalni tubuli) analizirani su protutijelima na AIF. Rezultati: Izražaj AIF-a nalazi se u kori, kao i u srži bubrega. U kori, AIF je uglavnom prisutan u proksimalnim zavojenim kanalićima (pct) dok je u srži uglavnom prisutan u sabirnim kanalićima (cd). Najveći postotak prisutan je u 2 tjednu DM pct skupine s 92% pozitivnih stanica. Cd pokazuje blago smanjenje pozitivnosti na AIF od 81% u kontrolnoj skupini od 2 tjedna do 89% u 2-mjesečnoj DM skupini. Intenzitet bojenja se povećavao od umjerenog do jakog bojenja. Slično smanjenje presutno je u pct, a intenzitet bojanja povećao se od blagog do jakog. Isti porast intenziteta zabilježen je u distalnim zavijenim kanalićima, ali relativno povećanje AIF pozitivnosti je bilo jače. Ono što je uočljivo je da je smanjenje i apsolutni intenzitet bojenja AIF-a otprilike isti od 2 tjedna do 2 mjeseca u kontrolnoj skupini i DM skupini, te da nema velike razlike između 2 tjedna i 2 mjeseca DM skupine. Apsolutna količina AIF pozitivnih stanica također je približno jednaka u skupinama DM i u dvjema skupinama od dva tjedna (38%), čak dvostruko veća u skupini DM od 2 mjeseca (18%). Zaključci: Budući da AIF ima dvostruku ulogu, za buduće studije je važno pronaći način razlikovanja AIF-a u različitim statusima, ako ih želimo usporediti. Ako se to može postići, AIF može postati parametar za procjenu stanja bubrega u DBB-a i eventualno ciljni alat za blokiranje napretka DBB-a.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:171:526457
Study programmeTitle: Medical Studies in English
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryMEFST Repository
Created on2018-10-09 12:03:54