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master's thesis
Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis
Split: University of Split, 2018. urn:nbn:hr:226:653500
Dujić, Andrijana
University of Split
University Department of Marine Studies

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Dujić, A. (2018). Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis (Master's thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:226:653500

Dujić, Andrijana. "Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis." Master's thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:653500

Dujić, Andrijana. "Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis." Master's thesis, University of Split, 2018. https://urn.nsk.hr/urn:nbn:hr:226:653500

Dujić, A. (2018). 'Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis', Master's thesis, University of Split, accessed 26 April 2024, https://urn.nsk.hr/urn:nbn:hr:226:653500

Dujić A. Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis [Master's thesis]. Split: University of Split; 2018 [cited 2024 April 26] Available at: https://urn.nsk.hr/urn:nbn:hr:226:653500

A. Dujić, "Utjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis", Master's thesis, University of Split, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:226:653500

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Metadata
TitleUtjecaj pH i temperature na sadržaj domoične kiseline u dagnjama Mytilus galloprovincialis
Title (english)Influence of pH and temperature on the domoic acid content in mussels Mytilus galloprovincialis
AuthorAndrijana Dujić
MentorIvana Ujević (mentor)
Committee memberJure Brčić (predsjednik povjerenstva)
Committee memberIvana Ujević (član povjerenstva)
Committee memberMirela Petrić (član povjerenstva)
GranterUniversity of Split
(University Department of Marine Studies)
Split
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Domoična kiselina (DK) je neurotoksin koji je polaran i topljiv u vodi, a uzročnik je amnezijskog trovanja školjkašima (engl. Amnesic Shellfish Poisoning - ASP). Kod ljudi se trovanje domoičnom kiselinom očituje gastrointestinalnim i neurološkim simptomima. U ovom radu koristila se metoda tekućinske kromatografije i masene spektrometrije (LC-MS/MS) za kvantitativno i kvalitativno određivanje domoične kiseline u mekom tkivu dagnje Mytilus galloprovincialis. Istraživan je utjecaj pH i temperature na stabilnost domoične kiseline u metanolskim ekstraktima. Otopina metanola se koristila za ekstrakciju domoične kiseline iz tkiva organizma. Rezultati pokazuju da temperatura ne utječe značajno na stabilnost domoične kiseline, dok pH vrijednost utječe pri niskim vrijednostima.
Abstract (english)
Domoic acid (DK) is a heterocyclic and water soluble neurotoxin that is responsible for Amnesic Shellfish Poisoning (ASP). The poisoning is characterized by gastrointestinal and central nervous system (CNS) clinical symptoms. In this research, the tandem method of liquid chromatography and mass spectrometry (LC-MS/MS) was used for the quantitative and qualitative determination of domoic acid in soft tissue of mussel Mytilus galloprovincialis. The influence of pH and temperature on the stability of domoic acid in methanol extracts was investigated. A methanol solution was used to extract the domoic acid from the shellfish tissue. The results show that the temperature does not significantly affect the stability of the domoic acid. Nonetheless, only low Ph values do affect its stability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:226:653500
Study programmeTitle: Marine Fishery
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka morskog ribarstva (magistar/ magistra inženjer/inženjerka morskog ribarstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public notePredmet: Toksičnost školjkaša
RepositoryRepository of University Department of Marine Studies
Created on2018-11-30 12:01:13